Development of thermostable Candida antarctica lipase B through novel in silico design of disulfide bridge
نویسندگان
چکیده
منابع مشابه
A novel self-activation mechanism of Candida antarctica lipase B.
Candida antarctica lipase B (CalB), resembling many other lipases structure-wise, contains a flexible lid that undergoes a surprisingly large conformational change when catalyzing hydrophobic substrates (e.g. triglycerides). Despite extensive and important applications in industry, it is so far still elusive whether CalB can be activated on a hydrophobic surface, like other lipases. From large-...
متن کاملRational redesign of Candida antarctica lipase B
This thesis describes the use of rational redesign to modify the properties of the enzyme Candida antarctica lipase B. Through carefully selected single-point mutations, we were able to introduce substrate-assisted catalysis and to alter the reaction specificity. Other single-point mutations afforded variants with greatly changed substrate selectivity and enantioselectivity. Mutation of the cat...
متن کاملFunctional expression of Candida antarctica lipase B in Eschericha coli.
Candida antarctica lipase B (CalB) is an important catalyst in bio-organic synthesis. To optimize its performance, either the reaction medium is changed or the lipase itself is modified. In the latter case, mutants are generated in Eschericha coli and subsequently expressed in fungal hosts for their characterization. Here we present the functional expression of CalB in the periplasm of E. coli....
متن کاملModelling of Enantioselectivity in Candida antarctica Lipase B
Enzyme selectivity towards a particular enantiomeric configuration of substrate is necessarily governed by chemical and spatial relationships between the substrate and the enzyme binding cavity. In the case of Candida antarctica lipase B experimental evidence shows that enantioselectivity of the enzyme towards the alcohol moiety 3-methyl-2-butanol varies significantly with respect to the chain ...
متن کاملImmobilization of Candida antarctica lipase B on Polystyrene Nanoparticles.
Polystyrene (PS) nanoparticles were prepared via a nanoprecipitation process. The influence of the pH of the buffer solution used during the immobilization process on the loading of Candida antarctica lipase B (Cal-B) and on the hydrolytic activity (hydrolysis of p-nitrophenyl acetate) of the immobilized Cal-B was studied. The pH of the buffer solution has no influence on enzyme loading, while ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biotechnology and Bioengineering
سال: 2011
ISSN: 0006-3592
DOI: 10.1002/bit.24371